University of Sao Paulo Medical School, Brazil
Monica V Marquezini is the Scientifi c Researcher at Pro-Blood Foundation of Blood Center of São Paulo since 1991. Is part of Experimental Air Pollution Laboratory-LPAE staff at the Department of Pathology, Medical School of University of São Paulo, Brazil, since 2010. Expert in Cellular and Molecular Biology with cell surface proteins studies and extracellular matrix research. Teacher of Cell and Molecular Biology themes at different graduation careers, Post-graduation advisor, and Coordinator of specialization courses and National and International Projects. Ph.D. in Science from the Federal University of São Paulo-School of Medicine UNIFESP-EPM (1996), M.Sc. in Molecular Biology UNIFESP-EPM (1987) and graduated in Biological Sciences Medical Modality from the University Methodist Piracicaba-UNIMEP, State of São Paul (1983).
Recent experimental data have provided associations between ambient PM2.5 (fi ne particulate matter; diameter ≤2.5μm) and propensity to infl ammation and chronic diseases especially among susceptible groups, such as elderly people. Th ere is cumulative evidence that type-2 diabetes mellitus is a chronic infl ammatory state aggravated by factors that promote endothelium infl ammation. Accordingly our hypothesis that the exposure of aged obese population to PM2.5 might aggravate type-2 diabetes, we tested this hypothesis in a model of diet-induced obesity where C57BL6 male mice were fed with regular chow (n=30; RC) or high-fat chow (n=36; HF) during one-year and randomly assigned to fi ltered (FA-RC, n=16; FA-HF, n=19) or PM2.5 concentrated air (600 μg.m-3) (EXP-RC, n=14; EXP-HF, n=17) daily during 1-hour for 30-days. Fast glycemia was measured before the animals were euthanized. All experimental procedures were approved by the Institution's Ethics Committee. Heart mRNA content of selected migration, signalization and adhesion proteins were measured by SYBR Green fl uorescence Real Time RT-PCR protocol using appropriate primers. Th ere were no diff erence between RC-EXP and RC-FA nor between HF-EXP and HF-FA body weight. Regarding fast glycemia, both, RC and HF groups, were diabetic, but only the HF group was aff ected by acute exposure to PM2.5 (mean±SD, EXP-HF vs FA-HF, 172.8±23.4 vs 156.7±17.6, p<0,05; EXP-RC vs FA-RC, 149.8±19.2, 139.7±15.3, ns; ANOVA). Th e expression profi le of the proteins studied, E-selectin, IL-6, VCAM-1, ICAM-1 and MMP-9, were diff erent in heart and lung. Proteins activated by infl ammatory stimuli involved in the inhibition of insulin signaling are being investigated.