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Biography

Mona Zaky Nasser has obtained her Master and MD degree from Cairo University. She has spent one year (2008-2009) as a Postdoctoral Researcher in Washington University in ST Louis, USA. She has been involved in the establishment of the molecular diagnostic unit in Beni-Suef University. Moreover, she is the Director of the quality assurance program in the clinical chemistry unit in Misr University for Science and Technology. She has supervised many Post-graduate candidates and participated in number of conferences where she has presented few posters and oral presentations. Her research interest includes molecular diagnostics and genetic background of human cancer.

Abstract

Introduction/Aim: Increasing evidence suggests thatseveral pathological hepatic diseases have been related to alterations of miRNAs expression.Th e present study was designed to assess the signifi cance of serum miR-21, miR-223, and miR-885-5p as potential biomarkers in diff erent clinico-pathological sequalae of HCV-related chronic liver disease. Patients and Methods: Serum miR-21, miR-223, and miR-885-5p were quantifi ed by real-time quantitative PCR in 60 Egyptian patients with HCV-related liver disease in addition to 25 healthy controls. HCV patients were classifi ed into: chronic HCV (n=15), liver cirrhosis (n=15), and hepatocellular carcinoma (HCC) (n=30). Results: Serum levels of miR-885-5p in cirrhotic patients (with or without HCC) were signifi cantly higher than the noncirrhotic patients; p=0.007 and healthy control; p=0.001. However, no such signifi cance was detected between HCV patients with and without HCC; p=0.12. Serum miRNA-885-5p was able to discriminate cirrhosis ± HCC from healthy controls using ROC analysis: AUC 0.85, 87% sensitivity and 80% specifi city. As regards serum miR-21, HCC patients had signifi cantly higher levels than non-HCC patients (non-cirrhotic and cirrhotic groups); p=0.048 and the control group; p=0.002. ROC could diff erentiate HCC from control group; AUC 0.89, 80% sensitivity, 80% specifi city. Serum albumin and bilirubin were signifi cantly correlated with miRNA-885-5p(r=-0.27, p=0.04) (r= 0.42, p=0.001) respectively, but such correlation was not observed with serum miRNA-21. In contrast, mi RNA 223 showed no signifi cant diff erence across the studied groups. Conclusion: Along the spectrum of HCV-related chronic liver disease, miR-885-5p could be a potential marker for advanced liver damage while miR-21 could be a helpful diagnostic marker for HCC.