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Biography

Trichosanthin (TCS) has been a midterm abortifacient traditional Chinese medicine for hundreds of years. In addition, TCS manifests a host of pharmacological properties, including anti-tumor activities. TCS has been reported to inhibit cell growth of a diversity of cancers, including cervical cancer, choriocarcinoma, and leukemia/lymphoma, etc. We explored the growth inhibition effect of TCS on lymphoma cells and its possible mechanism. Different doses of TCS were added to the cultured non-Hodgkin’s lymphoma (NHL) cell lines. The results showed that TCS could inhibit the proliferation of all NHL cells used in the experiment. And the growth inhibition activity was associated with the expressions of Mcl-1, Bcl-2 and Puma. Flow cytometric analysis disclosed that TCS mainly induced apoptosis. Furthermore, the TCS-induced apoptosis was attributed to the activations of caspased-3 and PARP-1. Therefore, TCS can inhibit NHL cell growth through inducing apoptosis, and the expression of Mcl-1, Bcl-2 and Puma maybe predicts its efficacy. These research progresses may provide an insight into cancer research and treatment as well as disclose new pharmacological properties of the ancient Chinese medicine.

Abstract

Trichosanthin (TCS) has been a midterm abortifacient traditional Chinese medicine for hundreds of years. In addition, TCS manifests a host of pharmacological properties, including anti-tumor activities. TCS has been reported to inhibit cell growth of a diversity of cancers, including cervical cancer, choriocarcinoma, and leukemia/lymphoma, etc. We explored the growth inhibition effect of TCS on lymphoma cells and its possible mechanism. Different doses of TCS were added to the cultured non-Hodgkin’s lymphoma (NHL) cell lines. The results showed that TCS could inhibit the proliferation of all NHL cells used in the experiment. And the growth inhibition activity was associated with the expressions of Mcl-1, Bcl-2 and Puma. Flow cytometric analysis disclosed that TCS mainly induced apoptosis. Furthermore, the TCS-induced apoptosis was attributed to the activations of caspased-3 and PARP-1. Therefore, TCS can inhibit NHL cell growth through inducing apoptosis, and the expression of Mcl-1, Bcl-2 and Puma maybe predicts its efficacy. These research progresses may provide an insight into cancer research and treatment as well as disclose new pharmacological properties of the ancient Chinese medicine.

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