Defence Institute of Physiology and Allied Sciences, India
Seabuckthorn (Hippophae rhamnoides L.) Elaeagnaceae, a unique and valuable plant has recently gained worldwide attention, mainly for its medicinal and nutritional potential. Three species of sea buckthorn namely Hippophae rhamnoides, Hippophae salicifolia and Hippophae tibetana are being cultivated in India, of which Hippophae rhamnoides L. ssp. Turkestanica is the main variety used commonly. Leaves of seabuckthorn are known to possess medicinal properties such as anti-oxidant, adaptogenic, wound-healing and anti-inflammatory activities. Very few studies have focused on identification and characterization of the bioactive components from Hippophae extracts, which is an important area for the development of Sea buckthorn based pharmaceuticals and nutraceuticals. The present study was undertaken to compare the total phenolic and flavonoid composition along with antioxidative properties of the alcoholic and aqueous extracts obtained from selected species of sea buckthorn leaves including sea buckthorn (Hippophae rhamnoides ssp. turkestanica) and willow-leaved sea buckthorn or sawthorn (Hippophae salicifolia L.). The characterization of the extract was carried out using marker compound, gallic acid by high performance thin layer chromatography and phytochemical analysis in terms of total phenol and total flavonoids. The total phenolic content was found to be higher in alcoholic extracts as compared to aqueous extracts. For both aqueous and alcoholic extract the gallic acid was found to be higher in Hippophae salicifolia L. than Hippophae rhamnoides L.