King Saud University, Saudi Arabia
Rashad Al-Salahi currently working at King Saud University, Saudi Arabia, and has 33 paper publications
Objective: This research was aimed to study and evaluate the antimicrobial activity of a novel 2-methylsulfanyl-[1,2,4] triazolo[1,5-a]quinazoline and its derivatives. Antibacterial activity of the target compounds was tested against a variety of species of gram positive bacteria as Staphylococcus aureus ATCC 29213, B. subtilis ATCC6633 and gram negative bacteria as Pseudomonas aeroginosa ATCC27953 and E. coliATCC 25922. In addition to some yeast and fungi as Candida albicans NRRL Y-477 and Aspergillusniger were screened. Methods: Antimicrobial tests were carried out by the agar well diffusion method, using 100 μL of suspension containing 1x108 CFU/ mL of pathologicaltested bacteria, 1x106 CFU/ml of yeast and 1x104 spore/ml of fungi spread on nutrient agar (NA), Sabourand dextrose agar (SDA), and potato dextrose agar (PDA) respectively. Key finding: The minimum inhibitory concentration (MIC) of the tested compounds has been determined by using broth double dilution method (Serially diluted technique) in proper nutrient. For comparison, ciprofloxacin and ketoconazole were used as antibacterial and antifungal reference drugs. Compounds 6, 9, 13, 14, and 11 were found to have the highest broad-spectrum antibacterial activity against StaphylococcusaureusATCC 29213, B. subtilis ATCC6633 and gram negative bacteria as Pseudomonas aeroginosa ATCC27953 and E. coli ATCC 25922 with MIC-values of 6.25 and 12.50 μg/ml. Conclusions: it is clearly that the synthesized compounds are promisingly significant, good antimicrobial agents. The present study revealed that compounds 6, 9, 13, 14, and 11 have been disclosed as potent antimicrobial agents and could be useful as templates for further development through modification or derivatization to design more potent antimicrobial agents.