S Rama Rao

S Rama Rao

North-Eastern Hill University, India

Title: DNA fi ngerprinting of the genus Cucumis using nuclear and chloroplast DNA sequences


Satyawada Rama Rao has obtained his M.Sc and Ph.D. degree from University of Jodhpur and taught at the same institution for more than 20 years before joining North-Eastern Hill University (NEHU), Shillong as Professor of Plant Biotechnology. He has published more than 55 papers in journals of International repute, and is presently a member of Research Policy Council of Indian Council of Forest Research and Education (ICFRE), Dehradun, India.


Nuclear ribosomal internal transcribed spacer (ITS) and chloroplast matk DNA sequences variation was analyzed to understand the inter-specific relationships of the genus Cucumis with focus on Indian representative species. 16 taxa, belonging to 11 species and 5 varieties of Cucumis were studied. The aligned ITS sequences were 688 bp in length, while that of matk sequences were 1364 bp. Both the regions showed variability with respect to sequence lengths, G+C content, nucleotide substitution, insertion/ deletion, transition/ transversion. Phylogeny reconstructions were conducted separately for these two data sets using maximum likelihood criteria and Bayesian inference. The genomic diversity and phylogenetic interrelations of the genus Cucumis will be discussed in detail.