S. Tsuyoshi Ohnishi

Philadelphia Biomedical Research Institute,USA

Title: Possible nutritional intervention of sickle cell diseases


S. Tsuyoshi Ohnishi is a profesoor and director of the Philadelphia Biomedical Research Institute at King of Prussia, University of Pennsylvania School of Medicine, Pennsylvania, U.S.A. His research are Brain ischemia and Diethyldithiocarbamate


When bloods from patients of sickle-cell disease (SCD) are deoxygenated in vitro, they sickle in a maple-leaf shape, but they do not form a sickle-shape cell. Nobody had paid attention to this phenomenon. We thought it was strange and studied about this carefully. Our findings were: (1) when we exposed the cells to 10 to 20 cycles of “dexoy-oxy cyclings”, nicely sickled cells were formed (2) When sickled cells were formed, the density of the cells increased by the Gardos effect (Calcium++-activated potassium+ efflux) (3) Using this method, we have shown that the formation of dense-cells was accelerated by the reactive oxygen species (ROS). This suggests that ROS scavengers may be used for nutritional intervention of preventing sickle-cell crises (4) If we repeated the cycling in the absence of an air-water interface, they formed only dense cells, but not the saickled cells