Deputy of General Director, Research Institute for Biological Safety Problems, Republic of Kazakhstan

Title: Live vaccine for equine influenza on the base of cold-adapted recombinant strain A/HK/Otar/6:2/2010


Dr.SandybayvNurlan received a PhD degree in microbiology in 2007. He is the Deputy General Director of the Research Institute for Biological SafetyProblems at the Ministry Education and Science of the Republic of Kazakhstan and the Principal Investigator for molecular biology of viruses at the Laboratory of Molecular Biology and Genetic Engineering.
Dr.Sandybayevhas authored and co-authored more than 172 scientific articles and abstracts, 7 books, and holds 34 author’s rights for his work on PCR methods, viral and bacterial strains, DNA and RNA sequencing and vaccine development.


A new strategy of vaccination for equine influenza (EI) is developing in last years in the western countries, especially in North America. Live vaccines from attenuated strain influenza virus are produced for more natural stimulation of immune systems. Immune responsecaused by natural infection,as opposed to immunity stimulated by inactivated vaccines, is more adequate and longer because of stimulation cellular and humoralimmune response.
The method of classical genetic is used for construction of recombinant strain for a candidate in vaccines. As donor HA and NA are used strain A/equine/Otar/764/07(H3N8) equine influenza virus actual for Kazakhstan and so high yield and cold-adapted strain A/Kong Hong/168/162/35 (H3N2) (phenotype ts+ and ca+). The basic scheme of receiving of reassortants is provides the following selective passages of parental viruses at presence of sera. The recombinant strain A/HK/Otar/6:2/2010 having HA and NA genes from the virulent strain and the internal genes from vaccine strain is result. Correspondence of genomic composition of reassortant is confirmed by PCR and sequencing. Absence of pathogenicity and toxicity on embryonated chicken eggs and the laboratory models are shown. The stability of reassortant genome is shown on more than 20 passages in the embryonated chicken eggs.
Live cold-adapted vaccine for prophylactic of equine influenza is produced on the base of strain A/HK/Otar/6:2/2010 (phenotype ts+ and ca+). Vaccine safety for foals, pregnant mares and adult horses is shown. The rates of humoral and cellular immunity of horse immunized by vaccine are studied. Expressed protective of vaccine challengeafter of horses by the virulent strain A/horse/Otar/764/07(H3N8) are shown. Animals of the vaccine challenge areprotected 100 % from disease. On duration of immunity, the twofold vaccination is protected from disease within 12 months.