Sara Mohebbi

Leibniz-Institute for Natural Product Research and Infection Biology, Germany

Title: In vivo monitoring the intracellular pH variation upon Aspergillus fumigatus infection in single apoptotic human monocytes


Sara Mohebbi is a research fellow and ph.D student in Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute (HKI), Jena, Germany. She earned her first M.Sc. degree in microbiology from Tehran science and researches center, (Tehran, Iran) and then attended to Uppsala University, (Uppsala, Sweden) and graduated from Cell and Molecular Biology Master Program. She published a part of her first master thesis as a research paper in a scientific journal. During her current research project, on 2013 she had a successful presentation in V International Conference on environmental, Industrial and Applied Microbiology, Madrid, Spain.


Aspergillus species, in the first place are ubiquitous fungi which ecologically tend to inhabit soil, water, vegetation and starchy products. Therefore they have an important effect on the global carbon and nitrogen reproduction chain. On the other hand, Aspergillus are well known opportunistic fungi that manipulate the immune system of human. Among the species, Aspergillus fumigatus is one of the most prevalent fungi which causes allergic forms of human disease and fatal aspergillosis. It has been previously shown that A.fumigatus has the following impacts on host cells: Once the conidia are inhaled, they would be phagocytosed by the epithelial cells of respiratory tract and localize into macrophages, developing aspergillosis. They consequently as same as the other infectious particles, lead the cell entering into apoptotic phase [1]. Also interestingly, the wild type melanotic conidia, can interfere with the intrinsic and extrinsic apoptotic pathways in macrophages and inhibit apoptosis[2,3]. Nevertheless the fact that apoptosis as a major consequent of infection is a pH-dependent process, still there is a lack of knowledge about the certain role of cytosolic pH during infectious diseases development. In this study the effect of melanin derived from A.fumgatus on pH variation during the apoptosis is hyperspectrally monitored in the human monocyte. Hyperspectral imaging (also known as imaging spectroscopy) is a research technique which its application is based on collecting and processing data from multiple fluorescent dyes in quantitative means. By applying this method, the emitted spectrum from multicolored fluorescent sample, is being divided into more extended bands and consequently, the visible and graphable images are provided.