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Sharda Choudhary

Sharda Choudhary

National Research Centre on Seed Spices, India

Title: Molecular studies of Cumin (Cuminumcyminum L.), for diversity and modeling

Biography

Sharda Choudhary, M A Khan and Balraj Singh Health & Beauty Secrets of Seed Spices ICAR- Ninional Research Centre on Seed Spices Ajmer

Abstract

Cumin (Cuminumcyminum L.) is a valuable seed spice belonging to the family Apiaceae. It is one of the oldest and economically most important seed spice with medicinal properties. Main countries producing cumin are India, Iran, Morocco and Guatemala. In the present study, experiments were conducted to derive the genetic relationship in Indian Cumin, as very little literature is available for molecular marker based genetic diversity especially in Cumin. Indian cumin germplasm accessions were assessed by molecular markers.This indicates very low level (47%) of genetic diversity among genotypes. Cluster analysis based on the resulting data was performed using UPGMA method and Dice's similarity coefficient by NTSYS software. The Computational studies for 3D structure and function of cumin protein, which showed homology with the protein domain of humans and E-coli illustrating that the database available on Apiaceae family is very low. Function analysis revealed molecular function of cumin as structural constituent of ribosome with 99.0% and oxidoreductase activity with 98.7% probability and biological process of oxidation-reduction and ion transport with a probability of 98.9% and 97.8% respectively.
Keywords: RAPD, PCR, Cumin, Seed Spice, genetic diversity, Polymorphism and DNA.

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