Smiti V. Gupta
Wayne State University, USA
Dr. Gupta is a tenured associate professor at the department of Nutrition and Food science, Wayne State University. She has created a successful and sustainable research program, with substantial external funding (>1M $) and a respectable publication record. This has been accomplished along with course and curriculum development, graduation of 5 PhD and 10 master’s students and close mentoring of a host of undergraduate students. Dr. Gupta developed a graduate level metabolomics course that later became the foundation of a 2 week long metabolomics workshop that she has teaches from time to time in Malaysia.
Lung cancer (LC) is the leading cause of cancer deaths in the United States. The objectives of this study were (1) to use the metabolomic approach to investigate potentialearly biomarkers for LC and (2) document the effect of curcumin on change in metabolomic profiles in LC model. For this, LC was induced by N-nitrosobis-2-(oxopropyl) amine (BOP) in a group of Golden Syrian hamsters while the control animalswere injected with saline. Both groups (BOP treated and control) were divided into twosubgroups, fed either a control or a 2% curcumin supplemented diet. Urine, collectedweekly was analyzed with a Varian 500 MHz nuclear magnetic resonance (NMR)spectrophotometer. Principal Component Analysis (PCA) of the Proton (1H) NMR spectral data showed significant (p < 0.05) separation between the BOP treated and control groups. The metabolites responsible for the differences between the groups, were identified and quantified using the CHENOMX NMR metabolite database. Of the metabolites (n=26) found to be significantly different (p<0.05) between the BOP treated and control groups, some, such as, glycine, sarcosine, and alanine are reported as increased in other cancer types such as prostate. These could serve as potential biomarkers of cancer in general. Others may represent possible biomarkers of LC progression, specifically. Interestingly, the metabolites found to be in higher in concentration in the BOP treated group were found to be reduced in the subgroup fed curcumin, bringing the metabolomic profiles of the curcumin fed animals closer to those of the controls.