Sumarno Reto Prawiro
Brawijaya University Malang Indonesia
Sumarno R.P has completed his Ph.D from Airlangga University Surabaya Indonesia. He got Diploma Medical Microbiology at International Medical Research Kualalumpur Malaysia in 1983. In 1989 he worked as a research student at Institute of Tropical Diseases at Nagasaki University Japan. He is the Head of Magister Biomedicine Program at Medical Faculty of University Brawijaya Malang East Java Indonesia. He has published more than 20 papers in local and international journals.
Shigellosis is a diarrheal disease and still a big problem in developing country. The best prevention is by using a vaccine but so far there is not any suitable vaccine. Molecule adhesion of bacteria can serve a basic component of vaccine. Protein sub-unit pili which has MW 7, 9 and 48, 9 kDa S. dysenteriae as a molecule adhesive. Rabbit Ileal Loop (RIL) model can evaluate bacterial diarrhea. With the same purpose used Mice Legated Ilea Loop (MLIL) model. The aims of the study is to clarify protein sub-unit pili which has MW 7,9 and 48,9 kDa S. dysenteriae as a molecule adhesion can show protection diarrhea by MLIL model. The study was conducted by post control study design. The method of study is MLIL The first group without immunization as a control. The second groups were immunized with protein sub-unit pili which has MW 7, 9 kDa S. dysenteriae. Third group was immunized protein sub-unit pili which has MW 48, 9 kDa S. dysenteriae. The last group was immunized protein sub-unit pili which has MW 7, 9 and 48, 9 kDa S. dysenteriae. The choice of adjuvant’s immunogenic was ISCOM. Data analisis use ANOVA and Tuckey test The result show for preventing moving solution from intestine to lumen was found in forth group four if compared with the control was the best. After that second and third groupe. The second and third groupe was not different. The MLIL test can be used for studying bacterial diarrhea in animal.
Speaker PPTsDownload PPT