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Biography

Tesfaye Z. Nigusse participated as application support for external vendor system, applications development, system analyst, and project management for internal developed applications, business process management/improvement, business intelligence data warehouse development, knowledge management practice, and quality assurance framework development using ISO/ITILV3 and develop research on best practice, and bench-marking activities for internal business process excellence and conducting training, demo presentation for customer and employees.

Abstract

Background & objectives: Several plant products have been tested and found to possess antileishmanial activity. The present study was undertaken to evaluate antileishmanial activity of methanolic extract of Aloe otallensis on the promastigot stage of Leishmania aethiopica and Leishmania donovani comparing to standard drugs and also tried to screen its phytochemical constitute. Methods: Phytochemical screening was done using the method mentioned by Evan and Trease on Methanolic extract of the exudates to the leaf of Aloe otallensis. The Extract was also evaluated for in vitro antileishmanial activity against Leishmania aethiopica and Leishmania donavan on the strain of L.aethiopica (LDC/134) and L. donovani (AM 563), which is found from the black lion hospital parsitology unit. The result was compared to standard drug of Sodiumstibogluconate, milfostin and paramomycin. Result. The extract has a good antileishmaniacidal activity with an IC50 of 0.041 μg/ml on L.ethiopica (LDC/134) and 0.123 μg/ml on L.donovani (AM 563). The experimental data shows that relatively it has better activity than paramomycin and milfostin but less activity than sodiumstibogluconate, which is given in Ethiopia as a first line drug. The data analyses was done by pad graph prison version 5 software after it was read by ELISA redder at the wave length of 650 nm. The phytochemical screening of the exudates of aloe otallensis showed the presence of phenol, alkaloid and saponin. Conclusion: The methanolic extract of exudete of Aloe otallensis has a good anti leishmanisis activity relatively to paramomycin and milfostin and this activity may be attributed to phenol, Alkaloid and saponin presnt in the plant. But it needs further analysis for the conformation of which constituent present in much concentration and to know which one have highest role