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Biography

Ting Xu got his Ph.D. in ecology from the China Agricultural University (CAU) in 2003 and since then he has been working at CAU as a faculty member. Now he is a full professor and he focuses his research interests on the development of immunochemistry technologies for food safety, environmental pollution and ecological toxicology. He has co-authored 4 chapters of books and more than 50 papers published in international journals. He holds 5 Chinese patents.

Abstract

Five phage clones that selectively bind to a neonicotinoid insecticide imidacloprid have been selected from a commercialized phage display library containing linear 7-mer randomized amino acid residues. A competitive enzyme-linked immunosorbent assay (ELISA) for imidacloprid was developed by using a clone L7-1 displaying peptides specific to the analyte. The half-maximum signal inhibition concentration (IC50) and the limit of detection (LOD) of the phage ELISA for imidacloprid were 82 ng mL-1 and 5.6 ng mL-1, respectively. This phage ELISA showed little cross-reactivities with the compounds structurally related to imidacloprid (<1%). The average recoveries of the phage ELISA for imidacloprid in soil and water samples were in a range of 74–96% and 82–91%, respectively. The simple phage-displayed peptide technology described here has been proven to be a convenient and efficient method for the development of an alternative format of ELISA for small molecules. Keywords: Neonicotinoid insecticide; Imidacloprid; Phage display; Peptide; Competitive phage ELISA

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