Yalavarthy Prameela Devi
Kakatiya University, India
Yalavarthy Prameela Devi obtained her M.Sc. in Zoology with Environmental Biology specialization and Ph.D. in Environmental Biology from S.V.University (India) in 1984. She is now working as Professor at Kakatiya University, India. She has 30 years of teaching and 34 years of research experience and has good knowledge in Environmental Biotechnology and environmenatal bioanalytical techniques. She is an Endeavour fellow of Australia 2010. She has published nearly 90 scientific research papers and presented over 100 talks at various national and international seminars, workshops, training programs and conferences. She got UGC research Award (India) in 2006. Professor Yalavarthy Prameela Devi’s research interests include the development of low cost field kits for environmental monitoring of toxic chemicals using biological principles, environmental toxicology, ecological restoration and environmental management, global climate change, screening of phytochemicals for drug development, stem cell therapies for neuronal and liver disorders. She teaches for postgraduate students and guides students for Ph.D. 15 students obtained Ph.D. under her supervision.
The talk is on biotechnological approaches for the development of low cost field kits for detection, separation, identification and quantification of toxic chemicals. Toxic chemicals can be detected, identified and quantified by various chemical and instrumental methods, but monitoring from the environment is cumbersome, time consuming and costly. Alternatively enzymatic methods could be used in the field for monitoring of these toxic chemicals because of their exceptional performance capabilities, which include high specificity and sensitivity, rapid response, low cost and user-friendly operation. The analysis can be done in the field in an hour time. The operation cost and time is relatively less as compared with the other instrumental methods. The principle of operation also is so simple that parascientific personnel can also operate. The main principle involved in this method is the biochemical reaction between the toxic chemicals and its inhibition caused on the specific enzyme. The methodology involved was selection, extraction and standardization of enzyme activity from different sources and their inhibitory studies in the presence of different toxic chemicals for detection, identification and quantification. Paper and thin layer chromatographic methods, using enzymes and chromogenic reagents were standardized for detection and separation of various toxic chemicals. Tablet method was developed for quantification of various toxic chemicals using the same principle. Standard graphs were prepared for quantification. Based on these methods, field kits were developed for detection, separation, identification and quantification of various toxic chemicals. These developed biotechnological methods were successfully used for monitoring of toxic chemicals.