Handong global University, South Korea
Yosep Ji has completed his Bachelor's and Master's degree from Handong Global University and continuing PhD in same university under Professor Wilhelm Holzapfel.
Remarkable progress in recent studies on gut microbiota and microbiome enabled new diagnostic approaches towards understanding human patho-physiological status, also with regard to metabolic diseases and especially obesity. Deeper insight both in taxonomical relevance and the functional role of gut microbiota provides a solid basis for new strategies for combating various human disorders such as obesity. In this study we have monitored the influence of different strains of putative probiotics on host weight change concomitantly with modulation of microbiota and/or the microbiome and host gene expression in a diet induced obese murine model. Obesity was induced in a C57BL/6 mice model using 60% high fat diet for 10 weeks and the influence of probiotics was monitored compared to the control (non-probiotic) group. Putative probiotics isolated from various Korean kimchi samples, with Lactobacillus rhamnosus GG as reference strain, were administered by oral gavage (1 x 108 CFU/day) during the intervention period. Different molecular methods were applied to monitor microbiota, the microbiome and the physiological impact on the host. Short chain fatty acid composition was quantitatively determined by gas chromatography. Lower weight gain was detected in groups receiving putative probiotics. Metabolites such as short chain fatty acids were quantitatively modulated in groups receiving putative probiotics compared to the control group. Obesity related biomarkers showed significantly different gene expression levels in adipose tissues and liver in the groups supplemented with putative probiotics. Overall, consumption of the putative probiotic strains had a significant impact on obesity related dysbiosis and on host weight gain.