Ako Kasei co.,Ltd, Japan
Yoshihiro Hataguchi graduated from Himeji Institute of Technology University (1995) and belongs to Technical development division of Ako Kasei Co., Ltd. One of the company’s management policies is to devote to healthy human life by producing a variety of high quality and effective foods and liquids with original technology. Yoshihiro Hataguchi, a section chief, is working to research and develop new goods based on deep-seawater which possess functional effect in vivo.
Objective: We evaluated the potential eff ect of high-mineral RDSW on tumor growth vitro and vivo. Materials & Methods: RDSW was prepared from deep-seawater off Muroto in Kochi. Th e deep-seawater was desalinated and conditioned to give 5 magnesium:calcium ratios of 1:2, 1:1, 3:1, 1:0 and 0:1 at hardness of 1000, expressed by 5 high-mineral RDSWs. Th e RDSWs and distilled waters were used as test and control waters, respectively. Tumor cells used were MKN-45 and Hela cells. Tumor cells cultured in RPMI1640 medium prepared and were subjected to MTT assay for cell viability. Th e animals (15 male nude mice in each 6 group) were allowed free access to test or control waters during experiment. Tumor cells were injected in the subcutaneous part of posterior cervical region. Two weeks later, the animals were sacrifi ced and tumors grown and sera were obtained. All tumors were subjected to western blotting (WB) with sera from all animals to evaluate the immunoreactivity and antibody profi le was compared among 6 groups. Based on the diff erences from antibody profi ling, noteworthy 50-kDa proteins were analyzed by LC-MS/MS. Results: Comparison with control, 62-79% (MKN-45) and 72-85% (Hela) in cell viability, 54-80% (MKN-45) and 32-74% (Hela) in tumor volume, were observed with statistical signifi cance. Th erefore, high-mineral RDSWs potentially possess a repressive eff ect on tumor growth. LC-MS/MS identifi ed 44 (MKN-45) and 22 (Hela) proteins as candidate determinants involved in this phenomenon. More investigations including cloning, preparation of fusion proteins and WB, turn to 9 (MKN-45) and 5 (Hela) proteins so far.