A P Vladimirov
Ural Federal University, Russia
A P Vladimirov graduated from the State University in Cheboksary in 1975. In 1986, he defended his candidate dissertation, and in 2002 the Doctoral dissertation. Since 1972, he has been working in the field of development and application of speckle - holographic methods of measurement and control. He worked at the Institute of Polymeric Materials (Perm) and Institute of Metal Physics (Sverdlovsk). Currently, he is working in three organizations: The Institute of Engineering Science of the Ural branch of the Russian Academy of Sciences, Ural Federal University, and Institute of viral infections (Yekaterinburg).
Previously, the theory of dynamic speckle-interferometry which allows to study intracellular phenomena was developed by the author. The theory has been successfully used by the author with colleagues to study the effects of herpes virus in three types of cultured cells. The purpose of this report is to familiarize colleagues with the specificities of the theory and application of the method. First briefly discusses the theory of the method, the basic formulae used in practice. It is shown that the time-average intensity at the observation point and the correlation coefficient of the area of images depend on the parameters characterizing macroscopic changes (shape of cells) and microscopic processes in cells. The experiment with thawed cells, where the shape changes of cells due to the movement and the passing of intracellular processes is discussed.