Indian Institute of Technology(BHU), India
Abha Mishra got her PhD in Biochemical Engineering in 2001 from School of Biochemical Engg., IIT (BHU). She joined the same School as a faculty in the year 2003 after a short stay at JUIT Waknaghat, India as Faculty and In-charge of Bioinformatics Department in the year 2002. She has guided 2 PhD and 28 MTech students till date. She has published more than 50 articles in various international and national journals.
Medicinal plants containing active chemical constituents with high antioxidant property play an important role in the prevention of various infectious diseases and have potential health benefits. Some of the most important bioactive phytochemical constituents of medicinal plants like Curcuma longa, Ocimum sanctum and Musa sapientum were studied in this project because they are ubiquitous and abundantly grown. They could be a fairly economical therapeutic agent for wound management as a pro-healer, as an antioxidants and have antimicrobial properties. Curcumin from C. longa, Eugenol from O. sanctum were extracted by column chromatography using different solvents. These were purified in dichloromethane solvent which was verified by TLC and HPLC. Leucocyanidin was also isolated by TLC and HPLC from Musa sapientum. Characterization of Curcumin and Eugenol was done by NMR. The aqueous extract of C. longa did not show any antimicrobial activity while ethanolic extract exhibited inhibition. Aqueous extract of O. sanctum also showed negligible zone of inhibition in case of bacterial growth while showed inhibition in fungal growth and ethanolic extract showed inhibition in both bacterial and fungal growth. Aqueous and ethanolic extract of M. sapientum showed good antimicrobial properties. The antimicrobial properties of these active compounds were substantiated by in silico method.