Weizmann Institute of Science, Israel
Alon Savidor completed his PhD in 2008 from the University of Tennessee and The Oak-Ridge National Lab, Tennessee, USA. He is a Staff Scientist in the de Botton Institute for Protein Profiling at the Nancy and Stephen Grand Israel National Center for Personalized Medicine. He has published more than a dozen papers and book chapters in reputed journals including Science, Nature and Cell.
Complete sequence determination of antibodies and unknown proteins is considered challenging using high-throughput technologies . We developed next generation protein sequencing (NGPS), a novel method for de novo, full-length protein sequencing in high throughput. We first present a proof-of-concept for NGPS by applying it to bovine serum albumin (584 amino acids), equine myoglobin (153 amino acids) and bovine fetuin (342 amino acids). Excluding leucine/isoleucine and glutamic acid/deamidated glutamine ambiguities, end to end, full-length sequencing was achieved with 99-100% accuracy. Using NGPS, we were also able to obtain complete and rapid amino acid sequencing of antibodies, including the variable regions without using a reference database. We anticipate that this new method will mark the beginning of database independent proteomic analysis.