Antonio Rafael Coelho Jorge
Federal University of Ceara, Brazil
Degree in Biological Sciences from the Federal University of Ceará (UFC), he worked at graduation in biochemistry area of protein, specifically in Proteome area. Master and PhD in Pharmacology from the Federal University of Ceará (UFC) in the area of Cardio-Renal Pharmacology and Toxicology, which operates in the kidney hormones (ANP uroguanylin, urodilatin) involved in NaCl regulation ingested in the diet and vascular effects and kidney of venomous animal poisons. He is currently a post doctoral by CNPq.
Guanylin (GN), uroguanylin (UGN), and the bacterial heat-stable enterotoxin (ST) peptides comprise a new family of cyclic guanosine 3’-5’ monophosphate (cGMP)-regulating agonist. Ingestion of a salt meal induces secretion of GN and UGN into the intestinal lumen, where they inhibit Na+ absorption and induces Cl-, HCO3- , and water secretion. Simultaneously, these hormones stimulate renal electrolyte excretion by inducing natriuresis, kaliuresis, and diuresis. The highly integrated mechanism allows the organism to maintain sodium balance by eliminating excess NaCl in the urine. However, their physiological regulation within the kidney has not been studied. The aim of this study was showing changes on daily metabolism and renal function of rat under high sodium chloride ingestion. Its effects were examined using wistar rats maintained for ten days in metabolic cages. Control group received only water, two more groups received 1% and 2% solutions of sodium chlroride. We daily analyzed urinary volume, weigh, and food and water consume. The renal function was evaluated using isolated perfused kidneys, in which the kidneys were perfused after ten days in metabolic cages, only with Krebs-Henseleit solution containing 6g% of a previously dialysed bovine albumine serum. All data were analyzed by ANOVA and Student t-test with level of significance set at *p<0,05. Rat’s weights of 2% group decreased after eighth day, compared with control group, while 1% group did not show significative weight lost. Urinary volume and water consume increased, in both treatments, from second day. Food consume did not show significative among groups. In isolated kidney both treatments increase perfusion pressure (PP). The renal vascular resistence (RVR), urinary flow (UF), glomerular filtration rate (GFR) and the osmolar clearance (Cosm) increased in the 1% group compared with control group, however decreased in 2% NaCl group. Treatment with 2% NaCl decreased the sodium (%TNa+, %pTNa+), potassium (%TK+, %pTK+) and chloride (%TCl-, %pTCl-). These results suggest that a high salt ingestion on diet promote significative changes on daily metabolism and the renal function of rats.