Arildo José Braz de Oliveira
Universidade Estadual de Maringá, Brazil
Arildo José Braz de Oliveira has completed his PhD in Organic Chemistry at the age of 34 years at State University of Campinas and postdoctoral studies at Federal University of Parana in Biochemistry and Molecular Biology Department, in Brazil. He has experience in Natural Products Chemistry and Biotechnology of Medicinal Plants, acting on the following topics: Production of primary and secondary metabolites in plant cell cultures, isolation and identification of natural bioactive substances as alkaloids, fatty acids and polysaccharides. He has published more than 25 papers in reputed journals and has been acting as a reviewer some of these.
This study discusses and shows as to determine the degree of polymerization (DP) of inulin, exemplified by samples obtained from roots of Brazilian medicinal plants as Stevia rebaudiana and Pfaffia glomerata, using different analytical methodologies as colorimetric methods, gel permeation chromatography coupled to multiangle laser light scattering and refractive index detectors (GPC/MALLS), nuclear magnetic resonance (NMR), gas chromatography coupled mass spectrometry (GC/MS), electron spray ionization mass spectrometry (ESI/MS) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). It will be shown that these techniques and others can be used together to give complementary information thus providing a more accurate estimate of the overall DP of the inulin like molecules.