Binay Kumar Raut

Kathmandu University of Medical Sciences, Nepal

Title: Cytogenetic investigations in bone marrow cancers and cancer predisposing chromosomal instability syndromes


Binay Kumar Raut is working as a Lecturer in Biochemistry at Kathmandu University School of Medical Sciences, Nepal. He is interested to conduct research in Biochemistry. He authored many publications which mainly concentrates prevalence of iron deficiency in Pregnant women and Oncogenes responsible for Bone Marrow Cancer.


Background: Leukemia is a neoplastic proliferation of hematopoietic cells. Leukemia can be classifi ed as either myelocytic (myeloid) or lymphocytic (lymphoid) depending on the lineage of the leukemic cells and chronic and acute depending on the clinical course. Aim: Th e aim of the present study was to detect structural abnormalities by using automated karyotyping (IKAROS) soft ware in Leukemia cancer (CML, AML, ALL and CLL) samples. Methods: Conventional cytogenetics: Peripheral Blood Culture (PBC) and bone marrow aspirate supplemented with mitogen Phytohemagglutinin (PHA) metaphase chromosomes were harvested aft er 72 hours for chromosome analysis. Image acquisition and analysis was performed by using automated karyotyping (IKAROS) soft ware based on GTG banding. Results: Philadelphia chromosome involving the reciprocal translocation between the long arms of chromosomes 9 and 22 (46, XY, t (9; 22)) was observed in majority of Chronic Myeloid Leukemia (CML) patients of the present study. Also a high number of chromosomal structural aberrations were observed in Fanconi Anemia (FA) patient, a chromosomal instability syndrome predisposing to cancer. Conclusion: Conventional cytogenetics can be the method of choice in diagnosis of genetic conditions like CML but for other bone marrow cancers more studies with high sample numbers need to be carried out for determination of clear cut markers.