Saint Louis University
Chunfa Huang has completed his PhD from Xiamen University and postdoctoral studies at Wake Forest University School of Medicine and University of Texas Southwestern Medical Center. He was highly trained in lipid metabolism and cell signaling as a graduate student and as a post-doctor. Over the decades, his research area focuses on defining novel signaling pathways that regulate lipid metabolism and that are associated with human diseases including cancer., and has published more than 50 papers in reputed journals.
Cholesterol plays an important role in cancer de velopment, drug resistance and chemoi mmuno-sensitivity. Statins , cholesterol lowering drugs, can induce apoptosis, but also negatively interfere wit h CD-20 and rituximab-mediated activity. Our goal is to identify the alternative targets that could reduce cholesterol levels but do not interfere with CD-20 in chemo immunotherapy of chronic lymphocytic leukemia (CLL). We used MEC-2 cells, a CLL cell line, and the peripheral blood mononuclear cells (PBMCs) from CLL patients, treated them with cholesterol lowering agents, and analyzed the effect of these agents on cholesterol le vels, CD-20 expression and distribution, a nd cell viability in the presence or absence of fludarabine, rituximab or their combinations. We found that MEC-2 cells treated with cholesterol lowering agents (BIBB-515, YM-53601 or TAK-475) reduced 20% of total cellular cholesterol levels, but also significantly promoted CD-20 surface expression. Furt hermore, treatment of cells with fludarabine, rituximab or their combinations in the presence of BIBB-515, YM-53601 or TAK-475 enhanced MEC-2 cell chemoimmuno-sensitivity measured by cell viability. More importantly, these cholesterol lowering agents also significantly enhanced chemoimmuno-sensitivity of the PBMCs from CLL patients. Our data demonstrate that BIBB-515, YM53601 and TAK-475 render chemoimmuno-therapy resistant MEC-2 cells sensitive to chemoimmuno-therapy and enhance CLL cell chemoimmuno-sensitivity without CD-20 epitope presentation or its downstream signaling. These results provide a n ovel strategy which could be applied to CLL treatment.