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Du Qing

Du Qing

The Institute of Medicinal Plant Development, China

Title: The Impression of ubiquinone (coenzyme Q) in pharmacological and clinical experiments

Biography

Duqing has completed his PhD at the year 2015 in the major of pharmacognosy from the institute of medicinal plant development ,Chinese academy of Medical Sciences(CAMS) and Peking Union Medical College(PUMC) and postdoctoral studies in the institute of genetics and developmental biology,Beijing,China before the end of 2015.I am the member of American Society of Plant Biologists and Chinese Society for Cell Biology.Moreover,I am a pharmacist and the member of Chinese Pharmacist Association. We have published more than 5 papers in the BMC and Chinese famous journals as the research group.

Abstract

Salvia miltiorrhiza Bunge is one of the most important Traditional Chinese Medicine materials and an emerging model medicinal plant. No information is available for gibberellin metabolism in S. miltiorrhiza. Through genome-wide prediction and molecular cloning, 22 candidate gibberellin metabolism pathway genes were identified. It includes a SmKO, two SmKAOs, six SmGA20oxs, two SmGA3oxs and eleven SmGA2oxs. The deduced proteins showed sequence conservation and divergence and were predicted to be located in the endoplasmic reticulum(SmKO, SmKAO1 and SmKAO2), plastid (SmGA20ox4, SmGA2ox1 and SmGA2ox5) and cytoplasm (others) , respectively. Gibberellin metabolism pathway genes exhibited tissue-specific expression patterns and responded differentially to exogenous GA3 treatment, indicating the complex feedback of significant up-regulation and down-regulation among different tissue-types and time-points in S. miltiorrhiza. Among the 22 identified genes, nine responded to yeast extract and Ag+-treatment in S. miltiorrhiza hairy roots. Moreover, tissue-specifically expressed splice variants were identified for SmKO, SmGA20ox3, SmGA2ox3 andSmGA2ox11, of which SmKOv1, SmGA20ox3v and SmGA2ox11v1 were GA3-responsive, suggesting the importance of alternative splicing in regulating GA metabolism. The results show tissue-specifically expressed, feedback-regulated, stress-responsive and alternatively spliced novel genes and reveal multiple layer regulation of GA metabolism and crosstalk between gibberellin metabolism and tanshinone biosynthesis in S. miltiorrhiza.