Galba Maria de Campos Takaki
Catholic University of Pernambuco
Galba Maria de Campos Takaki is PhD in Microbiology by Federal Paulista University-SP, Brazil/Newcastle upon Tyne University- UK (1984); Post-Doctor in Effect of Post-antibiotic - Faculté de Pharmacie, Université de Montpellier, France (1988); Effective partnership working in Medical Mycology Research Center (MMRC), Chiba University, Japan; Professor by Federal University of Pernambuco (1992), and actually Professor and Coordinator of the Nucleus of Research in Environmental Sciences and Biotechnology in Catholic University of Pernambuco; published 238 papers in reputed journals and has been serving as an editorial board in Asian Chitin Journal (ACJ), and Brazilian Journal of Microbiology (BJM), and supervised 114 students in Post-graduation Courses.
Biosurfactants are metabolites of microbial origin that have surface-active properties and possess high emulsifying capacity, consisting of metabolic products from microorganisms. The purpose of this study was evaluated the potential of filamentous fungi Cunninghamella elegans strains biosurfactant-producing in low cost medium. The biosurfactant was produced by submerged fermentation using a inorganic salt media (ISM), supplemented with renewable resources corn steep liquor (CSL) and waste frying soybean oil (WSO) as sources of carbon and nitrogen. The production was carried out using 200 mL of culture medium, pH 5.5 with 1% inoculum maintained at 150 rpm for 96 hours at 28°C. A factorial design of type CCRD 2² was used with 12 assays to evaluate the optimal condition for the biosurfactant production. The biosurfactant produced by C. elegans UCP 596 showed high surface tension reducing activity the 30.1 mN/m-1 in the assay seven (1.15% CSL and 8.75% of WSO), and high emulsification index of 76, 6% using motor oil burned in the central point (5.37% CSL and 8.75% of WSO).Similar results was obtained to C. elegans UCP 542 with high surface tension reducing activity the 29.6 mN/m-1 in the assay one (10% CSL and 15% of WSO) and high emulsification index of 93,3% using motor oil burned in the condition seven (1.15% CSL and 8.75% of WSO). The results confirmed the biosurfactants to thermal (5-120°C) and pH (2-12) stability with respect to surface tension reducing activity and to emulsification activity and tolerance under hight sodium chloride concentrations (2-10 %).