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Biography

Henryk Mach is a senior investigator in Bioprocess Analytical and Formulation Sciences, Bioprocess Research and Development at Merck Research Laboratories, Merck & Co., focusing on applications of biophysical methods in vaccine development. He is an author of over 40 professional papers, book chapters and patents that reflect his research interests in the spectroscopy, virus assembly phenomena, and computational methods. Before joining vaccine development area at Merck in 1990, he was a Research Associate in Prof. Middaugh's laboratory at the University of Wyoming. He received an MS degree in Biotechnology from Agricultural University and PhD in Biophysics from Jagiellonian University, both in Krakow, Poland.

Abstract

Rational design of antigen purification and formulation with adjuvants requires a thorough knowledge of intermolecular forces that affect their structural and colloidal stability. The biophysical techniques used to assess these forces and interactions constantly evolve, and often require custom modifications to achieve practical goals while conforming to the theoretical rigor. Examples of adapting existing theories to gain specific insights on vaccine pre- and post-injection stability will be given. The techniques adapted will include static and dynamic light scattering, electrophoretic mobility, near-UV derivative spectroscopy, variable speed analytical ultracentrifugation, differential scanning calorimetry and attenuated total reflectance Fourier-transform infrared spectroscopy. A case study where such expanded analytical techniques were used will be based on human papillomavirus virus-like-particle (VLP) development