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Holly Roberts

Holly Roberts

University of Birmingham, UK

Title: Development of modified ferrocenes DNA probes for electrochemical SNP sensing

Biography

Holly Roberts obtained a 1st Class Hons Masters Degree in Chemistry with Analytical Science from The University of Birmingham in 2013. Her final project was with Dr. J Bunch investigating polymer detection and identification for forensic applications using TOF-SIMS. Later she went on to start a PhD project with Prof. J H R Tucker researching DNA detection using ferrocene modified nucleic acids as an electrochemical reporter for DNA detection. She has won several awards for both poster and oral presentations including Best in Year Oral Presentation and Best Science Poster at a public outreach event. She is also an avid STEM ambassador.

Abstract

Ferrocene is used regularly as a redox reporter within DNA as it is easily manufactured within the lab, functionalisable, stable and has well defined electrochemical behaviour making it an excellent candidate for DNA sensing. We present a modified ferrocene unit for use as a DNA based surface bound electrochemical probe. Ferrocene modified with a base on each cyclopentadienyl ring has been synthesised and substituted for two of the sugar units in the DNA backbone using automated DNA synthesis. This allows for the unit to be placed as close as possible to the site of a DNA base variation or single nucleotide polymorphism (SNP) thus increasing the sensitivity of the probe. An FcNA octomer has also been synthesised and investigated using cyclic voltammetry with a view to using the functionalised ferrocene to produced nanowires and redox active nanomaterials. On a gold electrode, surface formation gives reproducible redox behaviour both single stranded and upon hybridisation. Investigations using Square Wave Voltammetry (SWV) suggest an unusual increase in current on binding to a complementary DNA strand due to the effect of increased molecular rigidity. There is also some variation between fully complementary and partially non-complementary DNA strands which shows early promise for SNP detection. The probe has shown good chemical and electrochemical stability, losing only 2% current signal over 150 scans. The ability to sense SNPs using an electrochemical group closely associated to the site of interest could give a sensitive, portable, reusable and rapid SNP detector leading to faster medical diagnosis.