Georgia Regents University, USA
Il-man Kim is an assistant professor at Georgia Regents University. He completed his Ph.D. at the University of Illinois and postdoctoral training at Duke University. He is working on multi-disciplinary research projects related to cardiac disease. Particularly he is studying G protein-coupled receptor-mediated signaling pathways. He was selected as a finalist for the American Heart Association (AHA) Katz Basic Research Prize. He has been awarded three AHA grants. He has served on the grant review committee of AHA and NIH as well as Medical Research Council in UK. He has served on the editorial board member of several cardiovascular journals.
MicroRNAs (miRs) are small, non-coding RNAs that function to post-transcriptionally regulate gene expression. First transcribed as long primary miR transcripts (pri-miRs), they are enzymatically processed in the nucleus by Drosha into hairpin intermediate miRs (pre-miRs) and further processed in the cytoplasm by Dicer into mature miRs where they regulate cellular processes following activation by a variety of signals such as those stimulated by beta-adrenergic receptors (betaARs). Initially discovered to desensitize betaAR signaling, beta-arrestins are now appreciated to transduce multiple effector pathways independent of G protein-mediated second messenger accumulation, a concept known as biased signaling. We previously showed that the beta-arrestin-biased beta-blocker carvedilol activates cellular pathways in the heart. Our recent data demonstrated in human cells and mouse hearts that carvedilol upregulates a subset of mature and pre-miRs but not their pri-miRs in beta1AR-, G protein-coupled receptor kinase 5/6- and beta-arrestin1-dependent manner. Mechanistically, beta-arrestin1 regulates miR processing by forming a nuclear complex with hnRNPA1 and Drosha on pri-miRs. Loss- and gain-of-function approaches in cardiomyocytes (CMs) and in vivo mouse hearts also uncovered that beta-arrestin1-regulated miRs increased CM survival by repressing apoptotic genes. Our findings indicate a novel function for beta1AR-mediated beta-arrestin1 signaling activated by carvedilol in miR biogenesis, which may be linked, in part, to its mechanism for cell survival. These results also suggest that miR-target pairs regulated by beta-arrestin1 may exert cardio protective effects.