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Biography

Im Fong, LAM, lecturer, registered medical technologist (MT), a doctoral candidate of Zinan University (China) . Master degree of Zinan University (China) in Faculty of Medicine and Bachelor degree of Kaohsiung Medical University (Taiwan), Technology for Medical Sciences. She was Medical Technologist and member of Infection Control Committee in Kiang Wu Hospital. Currently, she is lecturer of Macao Polytechnic Institute, primarily teach microbiology for nursing students. Her reaserch interest is in microbiology and Immunolgy.

 

Abstract

Background

Staphylococcus epidermidis is one of the normal floras that can be found on human skin. Normally, it will not cause disease. But in some situation such as catheters, dialysis treatment, implantation of artificial cardiac valve, joint prosthesis and intraocular lens can cause disease. Staphylococcus epidermidis can form biofilms on inorganic materials and get into the blood circulation; it undergo a phenotypic change resulting in increased antibiotic resistance.

Objectives

Analyze and compare the antibiotic susceptibility in the planktonic form using the standard minimum inhibitory concentration (MIC) and in a biofilm using minimum biofilm eradication concentration (MBEC).

Optimized the results of minimum biofilm eradication concentration (MBEC) by XTT reduction test.

Methods

13 clinical isolates of Staphylococcus epidermidis from central nervous cathether were collected. They were analyzed for their antibiotic susceptibility by using the standard minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC). Chi-square was used to compare the sensitivity results. XTT reduction test were performed on biofilm positive Staphylococcus epidermidis.

Results

3 of 13 clinical isolates are biofilm positive Staphylococcus epidermidis. The isolates were susceptible to antibiotics tested using MIC and lost its efficacy when the bacteria were grown in biofilm. The change in susceptibility was significant high for all antibiotics tested.

Discussion

Patient with sepsis or isolated of Staphylococcus epidermidis from central nervous cathether is long standing or not responsive to therapy, MBEC testing should be considered as a biofilm may be present. XTT reduction test can optimize and show the quantitative result of MBEC. And also, the newer antibiotics for Staphylococcus should be tested for their performance in a biofilm using the XTT method.