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Biography

Jan Tkac received his Ph.D. degree in biotechnology at the Slovak University of Technology (Bratislava, Slovakia) in 2000. He received his postdoctoral appointment at the Linkoping University (2001-2003), Lund University (2003-2006) and Oxford University (2006-2008). Currently, he works at the Institute of Chemistry, Slovak Academy of Sciences in Bratislava. He was/is a recipient of an Individual Marie Curie Fellowship (2003) and an ERC Starting grant (2012). His main research activities cover a microarray platform of detection of glycoproteins by lectins with the aid of nanotechnology. He has published 80+ scientific articles, reviews, and 4 book chapters having 1000+ citations.

Abstract

Integration of nanotechnology into the field of glycomics can address current limitations of fluorescent glycan and lectin microarrays [1,2]. Moreover, label-free transducing schemes especially when working in an ultrasensitive fashion can be applied in a reliable detection of low abundant disease biomarkers [3-5]. For example, electrochemical impedance spectroscopy applied in our studies allows to detect analyte molecules down to a single molecule level (i.e. aM level) if immobilization architecture of ligands (lectins or glycans) is controlled at nanoscale [6-8]. Utilization of such devices in serological glycoprofile of samples from people having some diseases, in analysis of cancer biomarkers, cancer cell lines and viruses will be discussed. Acknowledgement: The financial support from the Slovak research and development agency APVV 0282-11 is acknowledged. The research leading to these results has received funding from the European Research Council under the European Union’s Seventh Framework Program (FP/2007-2013)/ERC Grant Agreement No. 311532 and from the European Union’s Seventh Framework Program for research, technological development and demonstration under grant agreement No. 317420.