Slovak Academy of Sciences, Slovakia
Jan Tkac received his Ph.D. degree in biotechnology at the Slovak University of Technology (Bratislava, Slovakia) in 2000. He received his postdoctoral appointment at the Linkoping University (2001-2003), Lund University (2003-2006) and Oxford University (2006-2008). Currently, he works at the Institute of Chemistry, Slovak Academy of Sciences in Bratislava. He was/is a recipient of an Individual Marie Curie Fellowship (2003) and an ERC Starting grant (2012). His main research activities cover a microarray platform of detection of glycoproteins by lectins with the aid of nanotechnology. He has published 80+ scientific articles, reviews, and 4 book chapters having 1000+ citations.
Integration of nanotechnology into the field of glycomics can address current limitations of fluorescent glycan and lectin microarrays [1,2]. Moreover, label-free transducing schemes especially when working in an ultrasensitive fashion can be applied in a reliable detection of low abundant disease biomarkers [3-5]. For example, electrochemical impedance spectroscopy applied in our studies allows to detect analyte molecules down to a single molecule level (i.e. aM level) if immobilization architecture of ligands (lectins or glycans) is controlled at nanoscale [6-8]. Utilization of such devices in serological glycoprofile of samples from people having some diseases, in analysis of cancer biomarkers, cancer cell lines and viruses will be discussed. Acknowledgement: The financial support from the Slovak research and development agency APVV 0282-11 is acknowledged. The research leading to these results has received funding from the European Research Council under the European Union’s Seventh Framework Program (FP/2007-2013)/ERC Grant Agreement No. 311532 and from the European Union’s Seventh Framework Program for research, technological development and demonstration under grant agreement No. 317420.