Justin T Baca
University of New Mexico, USA
Justin Baca is a practicing Emergency Medicine physician who directs a research program centered on novel diagnostics and point-of-care testing. He completed his MD and PhD degrees at the University of Pittsburgh, and his clinical training at Massachusetts General Hospital and Brigham and Women’s Hospital in Boston. As a physician scientist, he uses his clinical experience to help design and implement new clinical tests for use at or near the point of care. His background in analytical chemistry and physics has allowed him to contribute to the earliest stages of sensor and test development.
Foodborn illness effects millions of US residents each year, and enterohemorrhagic E coli accounts for a large proportion of severe disease. Current food screening and testing protocols rely on traditional culture and PCR methods, and may take multiple days to confirm the presence of pathogenic bacteria in food products. We present a rapid, sensitive, reagent-free detection methodology based on a hand-held Surface Acoustic Wave (SAW) sensor platform, which has the potential to greatly shorten the time to detection of pathogenic food contaminants. This SAW biosensor can detect strain-specific enterohemorrhagic E coli at extremely low concentrations and within a few minutes. No added reagents are required, and the sensor opperates in a variety of media including buffered water, growth media, river water, and sewage effluent. This sensing methodology has the potential to disrupt current food screening methods by eleminating the need for relatively lengthy culture and PCR methods. This approach has the potential to greatly reduce the morbidity and mortality from outbreaks of hemorrhagic diarrhea. At the same time, this will save the agricultural industry millions of dollars by preventing or limiting food recalls. Here, we report on the optimization of the SAW biosensor for specific detection of O:157 E coli, and discuss progress in the detection of other important pathogens in foodborn illness.