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Biography

Dr. Khalid Khalaf Alharbi is an Associate Professor in Medical Molecular Genetics at King Saud University, Saudi Arabia. He is member of various socities including Saudi Society of Clinical Laboratory Sciences , Human genome organization, Arab Science and technology foundation and Association of Saudi Medical Technologists.

Abstract

The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe¬sis and secretion under conditions in which stress to the endoplasmic reticu¬lum is increased in β-cells. In this case-control study, we have selected the rs7612462 polymorphism within the UBE2E2 gene to identify in Saudi population with type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p < 0.05). All of the subjects used in this study were non-obese (25