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M Waheed Akhtar

M Waheed Akhtar

University of the Punjab, Pakistan

Title: Designing antigens for reliable serodiagnosis of tuberculosis

Biography

M Waheed Akhtar is currently a Professor Emeritus in University of the Punjab, Lahore, Pakistan. His current research interests include engineering cellulases and xylanases and their over-expression to construct a potent enzyme mixture for saccharification of pre-treated plant biomass. His group is also working on designing fusion antigens for a reliable serodiagnosis of tuberculosis. He has supervised research of several dozens of successful PhD graduates and published over 150 research papers.

Abstract

Antigens of Mycobacterium tuberculosis produce highly variable response in different tuberculosis patients. Thus detection of multiple antibodies is necessary to ensure reliability in serodiagnosis of tuberculosis. Fusion molecules consisting of fragments having epitopes from two or more antigens showing high sensitivity against all the corresponding antibodies would be helpful in achieving this objective. In silico analysis to examine positioning of the epitopes in the fusion molecules can be of great advantage in designing such constructs successfully. We have produced a series of truncated antigens and constructed fusion molecules from epitope regions of several M. tuberculosis antigens like PstS1, TB16.3, echA, HSPX, PE35 and FbpC1. Data obtained on the basis of antibody detection in hundreds of plasma samples of both the smear positive and smear negative tuberculosis patients showed that sensitivity of some of the antigens after truncation increased significantly. Some of the fusion molecules constructed showed sensitivities very similar to the expected combined sensitivity of the contributing antigens. The heat shock protein HSPX of M. tuberculosis not only exhibited full sensitivity but also resulted in soluble expression in E. coli in fusion with some other antigens. Construction of the fusion molecules, their expression at high levels, some in a soluble form and showing high sensitivity in detecting multiple antibodies seem promising for developing a reliable and cost-effective serodiagnosis of tuberculosis.