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Manabendra Sarma

Manabendra Sarma

Indian Institute of Technology Guwahati, India

Title: Low energy electrons induced damage to selected DNA fragments

Biography

Dr. Manabendra Sarma received his B.Sc. and M.Sc. degrees in the year 2000 and 2002 respectively from Goalpara College (A College Affiliated to Gauhati University) and Indian Institute of Technology Guwahati, Assam, India. After getting his M.Sc. degree, he moved to Indian Institute of Technology Bombay, Mumbai, India in 2002 to pursue his Ph.D. work under Professor Manoj K. Mishra. He obtained his Ph.D. degree in 2008 and subsequently joined in the Department of Chemistry at Indian Institute of Technology Guwahati, Assam, India as a senior lecturer in the same year. In the year 2011, he received the prestigious BOYSCAST Fellowship of India to work with Professor Lorenz S. Cederbaum of University of Heidelberg, Germany for a year. Dr. Sarma’s current research interests include development of new theoretical approaches to laser assisted control of chemical reactions and resonances in electron-molecule scattering. Currently Dr. Sarma is an associate professor in Chemistry at Indian Institute of Technology Guwahati, Assam, India.

Abstract

We have theoretically and computationally investigated the mechanism of low energy electron (LEE) induced DNA damages such as single strand breaks (SSBs) and glycosidic bond cleavage in some selected DNA fragments (Fig. 1) viz., 2¢-deoxycytidine-3¢-monophosphate (3¢-dCMPH) [Fig. 1(a)], 2¢-deoxycytidine-5¢-monophosphate (5¢-dCMPH) [Fig. 1(b)] and sugar-phosphate-sugar (SPS) [Fig. 1(c)]. In this regard, we have used electronic structure theory and our newly implemented local complex potential based time dependent wave packet (LCP-TDWP) approach. Results from our calculations show that in 3¢-dCMPH and 5¢-dCMPH DNA fragments SSB predicted near 1 eV whereas in SPS moiety it appears around 0.6 eV. Further, in case of SPS moiety there are two dissociation channels namely 3¢ C-O and 5¢ C-O bond lesions. Our calculations show that the activation energy barrier for 5¢ C-O bond dissociation is less than of 3¢ C-O bond dissociation pathway. It has also been found that the metastable anion formed after electron attachment to SPS moiety is more long lived (~40-55 fs) than that to 3¢-dCMPH and 5¢-dCMPH fragments (~18-20 fs). On the other hand, the glycosidic bond cleavage in 3¢-dCMPH moiety [Fig. 1(d)] requires higher activation energy than of the SSB in the same fragment and thus least preferred channel compared to SSB.

      (a)                             (b)                          (c)                       (d)

Figure 1: Some of the selected DNA fragments. For each fragment bond susceptible for cleavage is marked with an arrow.