AL Nahrain University, Iraq
Assist Prof/ Dr. Maysaa has completed her M.Sc. and PhD from Al Nahrain University, Baghdad, Iraq and was one of academic staff members of Biotechnology department and group leader of medical biotechnology research. She worked on molecular analysis of infectious disease, some of the virulence factors of the pathogens, and appling some of bacterial cell components as antitumor active compounds. In 2012 she conducted her postdoctoral training at Nanomedicine-labortory of Immunology and Molecular Biomedical Research (N-LIMBR), at Deakin University, Australia. Recently join the SRF-IIE (US) fellowship at the same institute (N-LIMBR) as academic visitor and her major field of interest are nanomedicine based drug delivery systems for therapy and diagnosis.
A total of 53 sputum samples were collected from patients suffering from respiratory tract infections attending different hospitals of Baghdad and Al-Anbar (Ramadi) governorates. From these samples only fifty bacterial isolates were obtained and identified. According to the morphological and cultural characteristics results showed that 14 bacterial isolates (28%) were belong to Streptocoocus spp., 26 bacterial isolates (52%) were belong to Staphylococcus spp., four bacterial isolates (8%) were pseudomonas spp., and six isolates (12%) were identified as Klebsiella spp. Then further steps have been proceeded for identification Streptocoocus isolates species by biochemical tests, API 20 STEP system and blood hemolysis patterns. Results of full identification showed that only three isolates (21.4%) were identified as Streptococcus pneumoniae, five isolates (35.7%) were Streptococcus pyogenes, two isolates (14.3%) were Streptococcus mutans, two isolates (14.3%) were Streptococcus sangarius, one isolate (7.1%) was Streptococcus fecalis, and one isolate (7.1%) was Streptococcus agalacticae. Results of the antimicrobial susceptibility showed that these isolates gave different patterns of sensitivity to different antibiotics. For molecular diagnosis of these S. pneumoniae isolates and to study the epidemiology source of these isolates, ten different oligonucleotide primers were used and results showed that the genetic similarity was 67.92% between N8 and N6, while it was found to be 52.27% and 53.76% between N11 and N6 and N11 and N8, respectively. The source of isolation of three isolates confirms these results because the isolates N6 and N8 were isolated from Baghdad governorate while the isolate N11 was isolated from Al-Ramadi city (Al-Anbar province).