liver fibrosis is the wound healing response to a variety of acute or chronic stimuli, for instance, viral infection, toxins, and metabolic diseases. The pattern of hepatic stellate cell activation provides an important framework to pinpoint sites of anti-fibrotic therapy. IFN exhibit a wide spectrum of biological activities in target cells including antiviral, immunomodulatory, anti angiogenic and growth-inhibitory effects. The antiproliferative effects of IFN are the rational basis for their use in the treatment of metastatic malignant melanoma and renal cell carcinoma. The food use of S.oleraceus is justified by the high content of vit.C, flavonoids, and phenolics. The previous studies discussed the antifibrotic effect of IFN and S.oleraceus individually on liver fibrosis but in this study, the combination treatment has been studied well in the induced liver fibrosis models. Using different immunoassays, Histopathology, colorimetric and PCR techniques the results obtained showed that TAA causes hepatic fibrosis by induction of free radical production and decrease cellular antioxidant stores. The different treatment ways including combination treatment group showed a significant inhibitory effect in targeting hepatic fibrosis by reducing oxidative stress, increasing the activity of antioxidant enzymes and by inhibiting HSCs inflammation, activation and proliferation through increasing the levels of anti-fibrotic co-transcription factor PPAR-γ and decrease the levels of the main HSCs fibrogenic cytokines TGFβ1 and PDGF-BB.

In conclusion, biochemical, molecular and histopathological findings demonstrated that the combination treatment improved the antifibrotic effect better than the individual one,  the prophylactic usage of  SE protected against hepatic fibrosis and  SE had no side effect.