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Nada M Melhem

Nada M Melhem

American University of Beirut, Lebanon

Title: Characterization of Norovirus in Lebanon among hospitalized children less than 5 years old

Biography

Dr. Nada Melhem joined the American University of Beirut (AUB) in 2009 after earning her doctoral and postdoctoral training at the University of Pittsburgh, Department of Infectious Diseases and Microbiology. She is an assistant professor of infectious diseases and microbiology at the Faculty of Health Sciences (FHS), an associate at the Department of Biochemistry and Molecular Genetics and a member of the Center for Infectious Diseases Research, Faculty of Medicine at AUB. Dr. Melhem is a virologist and immunologist representing FHS on the National Committee for Communicable Diseases and the Polio National Containment Committee, both hosted at the Ministry of Public Health. Dr. Melhem conducts international and national collaborative research on potential therapeutic vaccine models targeting HIV- 1 and HIV drug resistance in Lebanon, respectively. She has designed and implemented projects on viral hepatitis as well as HIV and viral hepatitis co-infections among high-risk groups. Dr. Melhem has published on HIV and viral hepatitis and is currently assessing the prevalence of Norovirus among hospitalized children less than 5 in Lebanon and the genotypic characterization of the virus.

Abstract

Norovirus (NoV) is responsible for at least 50% of all gastroenteritis outbreaks worldwide. NoVs are classified into six different genogroups (GGI- GGVI) based on the viral capsid protein with NoV genogroup II genotype 4 (GGII.4) being the predominant strain causing human diseases. To our knowledge, no data exist on the burden of NoV in Lebanon among hospitalized children less than 5 years old. Written informed consents were obtained from the legal guardians of hospitalized children and consequently stool samples and medical data were collected. A standardized questionnaire including demographic, epidemiologic and clinical observations was used at the time of hospitalization of children presenting with diarrhea. A total of 739 eligible stool samples were collected from six major hospitals in Lebanon. Viral RNA extraction was performed followed by reverse transcription using genogroup-specific primers for GI and GII genotypes Nucleotide sequencing of NoV positive samples was performedusing the PCR primers. Multiple sequence alignments were carried out and phylogenetic trees were constructed using the MEGA 6 software. A total of 83 norovirus cases (11.2%) were detected over the course of 2 years. The mean age of these cases was 16.2±9.5 months. The incidence of NoV infection was the highest during the summer season (June-August) of each year. The majority of the NoV cases were NoV genogroup GII (n=78) with a total incidence rate of 10.6% while 5 samples tested positive for NoV genogroup GI with a total incidence rate of 0.7%. Most samples showed positive symptoms of diarrhea (95.1%) followed bydehydration (89.0%), vomiting (76.8%) and fever (67.1%). The majority of the isolated cases were caused by GII.4a and GII.4b (67%) with isolated cases of GII.1, GII.3, GII.6, GII.7, GII.9, GII.13 and GII.21. Similarly, GI.3 and GI.4 were detected. Our results are similar to those detected in the developed countries. We are in the process of designing seroprevalence data to assess the level of protection following infection with NoV.

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