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Nagwa Meguid

Nagwa Meguid

National Research Center, Egypt

Title: Expression of ROS related transcripts in Egyptian children with ASD

Biography

Dr Meguid holds her Ph.D. from Alexandria University. She is a professor of human genetics at the National Research Center (NRC) in Egypt. She is a Senior Geneticist at the Genetics Institute, California and Yale University; a fellow of Uppsala University, Sweden. She was selected to win the outstanding L’Oreal UNESCO Award for women in Science for Africa & Middle East (2002). She was given the National Award for Scientific Excellence in Advanced Technology in 2009. NRC Appreciation Prize in Medical Sciences, 2011.She is the Founder of Autistic disorders Clinic in the NRC. She is the head of the laboratory of research in DNA and biochemical changes in genetic disorders. She is a member of the International Jury L’OREAL-UNESCO Awards « For Women in Science » 2008 - 2015. She supervised around 70 Ph. D. &and master theses. She has more than 100 publications. Peer Reviewer for Scientific Journals.

Abstract

Autism Spectrum Disorders (ASDs) have become more spread wide over the recent years. In the present study we attempt to unveil the association between ASD and mediators of oxidative stress pathway at the molecular level. We used pathway focused PCR array to analyse gene expression pattern of 84 oxidative stress transcripts in peripheral blood mononuclear cell (PBMC) pools isolated from a total 28 patients with mild/ moderate or severe autism and 16 non-autistic healthy subjects (each sample is a pool from 4 autistic patients or 4 controls). All the participants were diagnosed using; Fourth Edition, Text Revision (DSM-IV-TR), Childhood Autism Rating Scale (CARS) and Autism Diagnosis Intervention- Revised (ADI-R) were done. Only 8 genes showed differential regulation over 1.5 fold change accompanied by statistical significance (p < 0.05) when compared the autistic group to the non-autistic one. The transcriptional profile revealed down regulation of 7 transcripts : Ferritin heavy polypeptide1, Glutamate-cysteine ligase modifier subunit, Neutrophil cytosolic factor2, Prostaglandin-endoperoxide synthase 2, Prion Protein, Superoxide dismutase2 mitochondrial, and Thioredoxin; and up regulation of one gene Glutathione peroxidase 7 in the PBMCs of autistic patients (either mild or severe) compared to controls (p < 0.05 for all). These results suggested that ASD is accompanied by dysregulation of the molecular signals involved in oxidative stress pathway. The current data form the bases for focused studies using single gene expression or custom arrays on a larger number of cases to get the most statistically regulated factors. Acknowledgements for the STDF project for financial support.