Pedro Henrique Sá Costa

Pedro Henrique Sá Costa

Federal University of Ceara, Brazil

Title: Modulation of guanylin pathway by enalapril in 5/6 nephrectomized rats


Pedro Henrique Sá Costa is graduated in Pharmacy, and Master in Pharmacology from Federal University of Ceará. Currently, he is a PhD student linked to the Department of Physiology and Pharmacology of the Federal University of Ceará, where he researches the theme of experimental chronic kidney disease and natriuretic peptides.


Chronic kidney disease (CKD) is characterized by loss usually slow, progressive and irreversible of renal function. In this context, more studies are necessary for establishment of a link between DRC and regulation of natriuretic peptides, as guanylin (Gn), uroguanylin (UGn) and atrial natriuretic peptide (ANP), and the effect of angiotensin II (Ang II) on regulation of these peptides. Thus, we sought to evaluate a possible modulation of the guanylin by enalapril in the 5/6 nephrectomy model (nx5/6). We used male Wistar rats, weighing between 250-300g. The animals were divided into 4 groups (n = 8): untreated control group treated or not with enalapril (10 mg/kg oral) (SHAM and SHAM+E) and group subjected to nx5/6 treated or not with enalapril (10 mg / kg oral) (Nx and Nx+E). Kidney samples were sent for histological analysis and evaluation of mRNA expression of Gn, UGn, ANP and membrane guanylate cyclase receptors, GC-A and GC-C, and the clearance receptor (NPR-C). Nx showed increased intrarenal gene expression of Gn (Nx=13.92 ± 5.13; SHAM=1.08 ± 0.20) UGn (Nx= 12.77 ± 7.00; SHAM=1.04 ± 0.13), GC-A (Nx=5.91 ± 1.36; SHAM=1.06 ± 0.17) and NPR-C (Nx=7.84 ± 1.72; SHAM =1.15 ± 12.27), and Nx+E had reduced genes for UGn (Nx+E= 0.10 ± 0.03; Nx = 1.75 ± 0.96), GC-A (Nx + E= 0.031 ± 0.01; Nx= 1.18 ± 0.27) and NPR-C (Nx+E= 0,03 ± 0.01; Nx= 1.8 ± 0.24) when compared to Nx. Together, these data suggest a hyperactivation the path of guanylin in CKD, and modulation of this peptide class by Ang II.