Rafael Silva Torres

Rafael Silva Torres

Instituto Politecnico Nacional, Mexico

Title: Prunus serotina Erth rich in fl avonoids and coumarins, is cytotoxic to the tumor cell line Hela


Rafael Silva Torres has completed his PhD. at the age of 57 years from EscuelaNacional de CienciasBiológica of National Polytechnic Institute and abroad studies M. Phil. from Loughborough University of Technology Great Britain and sabbatical year from Museum National D`HistoireNaturelle Paris France. He has published more than 14 papers in reputed journals and 1 book chapters and hasbeen serving as an editorial board member of repute journals. He was director of 45 Bsc. Thesis and he was participated in more than 150 national and international congress. He is membership of National Association of Pharmaceutical Sciences and American Chemical Society. He is investigating the properties antitumor of medicinal plants such as: Sedum praealtum DC., PrunuaserotinaErth and Hylocereuspolyhizus.


There is great diversity in Mexico and many fl ora and faunaspeciesthat remain to be studied. One example is Prunusserotina Erth. Anethanolic extract from the fruit was prepared, in its chemical composition showed a lot of secondary metabolites. Besides, since cancer is adisease with high incidence rate and mortality, there is much interest in developing new treatments for chemotherapy or chemoprotection, based in Mexican plants. In Mexico, breast and cervical cancer stand out with high fi gures of impact and mortality. Cancer is defi ned as the alteration of cell cycle, resulting of uncontrolled proliferation and inhibition of apoptosis. Certainly, secondary metabolites from plants may perform as inhibitors of proliferation or directly as cytotoxic molecules, inducing apoptosis or necrosis in tumor cells.A probable antitumor eff ect of the fruit from Prunusserotina Erth (locally named capulín), is currently being conductedin our labs, using the HeLa cells in vitro model. Th e ethanolic extract from the fruit was further chromatographed on a dry silica gel column. Th e fractions were chemically and biological tested for their content. Th e extract and fractions were used for treatments to the HeLa cells in vitro and the cytotoxic eff ect was initially observed by inverted optic microscopy and death was estimated by fl ow cytometry techniques. Th e fractions were obtained using 80-20 % methanol-acetone and numbers 3 and 4 tested positive for alkaloids, fl avonoids and cumarins. Both showed cytotoxic eff ectand the fractions had better cytotoxicity than the ethanolic extract.