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Sangeeta Yadav

Sangeeta Yadav

Babasaheb Bhimrao Ambedkar University, India

Title: Detection of quorum sensing molecules during biofilm mediated degradation of lignin

Biography

Dr. Sangeeta Yadav is working as Young scientist at Deptt. of Environmental Microbiology, B. B. Ambedkar Central University, Lucknow, India. She has completed his PhD in Bioscience and Dr. D.S.Kothari post doctoral fellowship on the lastest issue of environmental microbiology and biotecnology. She has also awarded CSIR- RA, CSIR-Senior Research Fellow (SRF), and Junior Research Fellow (JRF) During her doctorial degree, she got Gold medal for best research paper presentation in National Conference. She has published several (23) national and international research papers of high impact factor in area of bacterial degradation and management of Post Methanated Distillery Effluent (PMDE) for environmental safety. She has also published a book as co-author, 6 scientific Hindi articles and 6 book chapters. Her work has been widely cited and published by mass media. She is also life member of various scientific societies like Biotech Research Society of India (BRSI), Trivandrum, India; Academy of Environmental Biology, Lucknow, India; Association of Microbiologist of India (AMI), India; The Indian Science Congress Association (ISCA), India, and Society of Toxicology (STOX), India. Currently she is working in the area of Bioremediation and Metagenomic.

 

Abstract

Biofilm mediated bioremediation is a promising technique to treat the complex compounds and biofilm formation is governed by quorum sensing. Lignin is a major component of wood and coloring recalcitrant compound which creates problem during degradation of lignin containing wastewater discharged by the wood based industries like pulp and paper mill industries. A kraft lignin-degrading potential bacterium showed biofilm formation and lignolytic enzyme activity was isolated from sludge of pulp and paper mill and characterized as Enterobacter cancerogenus by 16SrRNA gene sequencing. The E. cancerogenus showed production of name of AHL as quorum sensing molecules and production of EPS which protect the bacteria from adverse condition. However, this strain reduced the color (78%) and lignin content (68%) from kraft lignin-mineral salt medium when supplemented with glucose at pH 7.8 and 30°C after eight days. Functional groups of lignin are degraded effectively by E. cancerogenus proved by Fourier transform infrared spectroscopy, HPLC and gas chromatography-mass spectrometer, and the degradation products were amides, alcohols and acids. Microscopic analysis revealed that biofilms formed in presence of lignin accumulate higher biomass with greater thickness compared to the biofilms produced in presence of glucose as sole carbon source. Meanwhile, the genotoxicity of degraded lignin determined by examining Allium cepa root cell division showed reduced chromosomal aberration. Thus, this study concluded that this strain may be useful for decolorization of industrial wastewater containing high concentration of lignin.