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Biography

Sarah Zecchin is a microbial ecologist, specialized in the analysis of environmental microbial communities using Next-Generation Sequencing techniques. She graduated in Biology as a bachelor and master student in 2010 (University of Padova) and in 2013 (University of Milano-Bicocca) respectively. She started working on rice rhizospheric sulfate-reducing bacteria during her master traineeship at the Division of Microbial Ecology at the University of Vienna (Austria). During her PhD (obtained in 2017) in Food Systems at the University of Milano, she studied the rice rhizospheric microbial communities involved in arsenic contamination. She is currently working as a postdoctoral researcher at the Department of Biology at the University of Konstanz (Germany), dealing with novel species of sulfate-reducing bacteria living in rice rhizosphere. 

Abstract

Rice is one of the crops with highest levels of arsenic (As) contamination. Italy, the first European rice producer, is affected by this health issue. The European commission recently established specific limits for As content in rice grain (Commission regulation (EU) 2005/1006). Continuous flooding, commonly employed in rice cultivation, has been identified as the main factor inducing high solubilisation of As from soil minerals and consequent uptake by the plant. Microorganisms influence As biogeochemistry both directly, by converting different oxidation states of the metalloid or by methylation, and indirectly, acting on As containing soil minerals. Since little is known on the actual role of rhizosphere microorganisms in driving As contamination in rice, our work aimed at deciphering how water managements influence the development of bacterial populations involved in the biogeochemical cycle of As, thus determining As contamination of rice grain. High throughput sequencing, PCR-based as well as microscopy methods were applied to study the microbial communities inhabiting different rhizosphere compartments of rice. We demonstrated that under continuous flooding, iron-reducing and sulfur-oxidizing bacteria emerged, explaining As solubilization from iron- and sulfide-containing minerals. Moreover, As-methylating bacteria were enhanced, resulting in organic As accumulation in rice grains. On the other hand, As- and iron oxidizing bacteria were enhanced in limited water usage condition (aerobic rice), where rice grains contained negligible As concentration. In this study, a direct involvement of specific rhizosphere microbial populations in determining As fate in rice paddy soil was assessed. Agronomic practices promoting beneficial rhizosphere population should be further refined in order to achieve the safety requirements for rice grain production.

Figure 1 Effect of two different water regimes (continuous flooding and aerobic rice) on rhizospheric microbial populations and on As content in rice grains.