Vitthalrao Khyade is working on Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Pune, India. He is also a member of Agriculture Development Trust, India.


The honey bees are well known for their behavior for effective communication. The biogenic amines exert a significant influence on the behavior in insects like honey bees. The present attempt is concerned with analysis of the levels of biogenic amines, like octopamine, dopamine and serotonin in the brain of worker honey bee, Apismellifera (L). The bioassay of the amines was carried out through the High Performance Liquid Chromatography with electrochemical detection. The influence of various factors on the levels of octopamine, dopamine and serotonin was analyzed. Exiting foragers were stressed by clamping their legs , which resulted in significant increase in the level of octopamine and serotonin after ten minutes. The seasonal variations in the levels of the three amines were found observed for the sampled from the colony during spring, summer and autumn. The levels of the three amines were highest during June – September, which correspond to high levels of colony foraging activity. The variations in the levels of the amines may reflect seasonal changes in colony nutrition, population size or brood rearing activities. In comparison with the randomly aged worker bees, the newly emerged bees were found with significantly lower levels of the three amines in their brain.
Keywords: Biogenic amines, Apis mellifera, stress, cerebral ganglia.