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Ya-Jun Yang

Lanzhou Institute of Husbandry and Pharmaceutical Science of Chinese Academy of Agricultural Sciences, China

Title: UPLC-Q-TOF/MS-based urine and plasma metabonomics study on the ameliorative effects of aspirin eugenol ester in hyperlipidemia rats

Biography

Jian-Yong Li is a Leader of Research and Development program at Agricultural Science and Technology Innovative Program of CAAS. He has obtained his BSc degree in Pharmacy (1995) from Lanzhou University, MS degree in Clinic Veterinary Sciences (2000) from the Graduate School of CAAS and his PhD degree in Analytical Chemistry (2005) from the Graduate School of CAS. He was awarded the Research Medal of the nation government in 2009 and 2011 for his work on new animal drugs. Currently, his research is on research and development of novel animal drug, including chemistry, phamacokinetics, pharmacology and toxicology of pharmaceuticals.

Abstract

The main objective of this study was to investigate the ameliorative effects of AEE in hyperlipidemic rat. After five-week oral administration of aspirin eugenol ester (AEE) in high fat diet (HFD)-induced hyperlipidemic rats, the impact of AEE on plasma and urine metabonomics was investigated to explore the underlying mechanism by UPLC-Q-TOF/MS analysis. Blood lipid levels and histopathological changes of liver, stomach and duodenum were also evaluated after AEE treatment. Without obvious gastrointestinal (GI) side effects, AEE significantly relieved fatty degeneration of liver and reduced triglyceride (TG), low density lipoprotein (LDL) and total cholesterol (TCH) (P<0.01). Clear separations of metabolic profiles were observed among control, model and AEE groups by using principal component analysis (PCA) and orthogonal partial least-squares-discriminate analysis (OPLS-DA). 16 endogenous metabolites in plasma and 18 endogenous metabolites in urine involved in glycerophospholipid metabolism, fatty acid metabolism, fatty acid betaoxidation, amino acid metabolism, TCA cycle, sphingolipid metabolism, gut microflora and pyrimidine metabolism were considered as potential biomarkers of hyperlipidemia and be regulated by AEE administration. It might be concluded that AEE was a promising candidate for hyperlipidemia treatment. These findings could contribute to the understanding of action mechanisms of AEE and provide evidence for further studies.