Jen-Ai Hospital, Taiwan
Yen-Chein Lai has served as an Assistant Professor in the School of Medical Laboratory and Biotechnology of Chung Shan Medical University (CSMU) since 1998. She earned her B. A. degree in the Department of Medical Technology of the National Taiwan University College of Medicine (NTUMC) in 1989 and Ph.D degree at the Institute of Biochemistry of NTUMC in 1996. Her PhD research focused on mechanism analysis of HDV ribozyme activity. She has had much experience in the teaching of Molecular Diagnostics and Clinical Chemistry courses. Currently, her research aims are the molecular-genetic analysis of patients with certain cancer syndromes.
Granulosa cell tumors are rare ovarian malignancies. Their characteristics include unpredictable late recurrent and malignant behavior. Recent molecular studies have characterized the FOXL2 402C > G mutation in adult-type granulosa cell tumor. In this study, we report an 80-year-old woman with a granulosa cell tumor arising from ovary. She presented with a huge pelvic mass with postmenopausal bleeding. No obvious intraperitoneal tumor implants were observed during operation. Final diagnosis was granulosa-theca cell tumor without capsule invasion. No recurrent disease was noted during 3-year post-operation follow-up period. Molecular studies showed a heterozygous FOXL2 402C > G mutation in the tumor by direct gene sequencing. In addition, DNA replication error, on analysis of the lengths of CAG repeats in androgen receptor gene, revealed defective DNA mismatch repair system in the granulosa cell tumor. We propose that the 402C > G mutation in FOXL2 is critical to the development of adult granulosa cell tumor. However, the malignant behavior of this tumor is driven by DNA mismatch repair deficiency. Unequal DNA copy numbers were noted on array comparative genomic hybridization. This implies that there is malignant potential even in the early stage of the granulosa cell tumor. Late malignant recurrence may be a late event of DNA repair function disability, not directly related to pathognomonic FOXL2 mutation.