Federal University Dutse-Nigeria
Oil Producing as well as consuming nations of the world must rise up to the challenge of environmental pollution due to spillage of both crude and refined hydrocarbon products. The devastating effect of crude oil on both living and non living components of the environment is something that deserves serious attention. For a cost-effective and sustainable restoration of the nonliving environment, some wasted materials were assessed in this work. Experiments were conducted to determine the nutritional contents of some selected wastes with a view to assessing their effect in bacterial degradation of Bonny light crude oil. Samples of sewage sludge, Chicken droppings and cow dung were obtained and processed. Crude protein, fat, fibre, ash, Phosphorus and trace metals contents of the wastes were determined using standard procedures. The results obtained revealed that the wastes contained appreciable essential nutrients like Nitrogen, Phosphorus, Magnesium, Manganese and Zinc. Significant difference (P<0.05) between the wastes was noted in all the determinations made except in Phosphorus content. Soil and water samples were collected from the garden farm. Crude oil-degrading bacteria were isolated from these samples using selective enrichment technique. The isolates included Bacillus megaterium and Bacillus licheniformis. The bacteria were used in crude oil degradation experiment. The residual crude was extracted after degradation and subjected to chromatographic analyses using GC-MS methods. A significant reduction (P<0.05) in heavy metals, Total Petroleum Hydrocarbons and Polycyclic Aromatic Hydrocarbons was achieved.