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Biography

Zarina Arif, has completed her Ph.D. in the year 1993 from Aligarh Muslim University, India. She has work as Research Assistant (23-9-1993 to 22-7-2008) at the College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia. It was research cum teaching job. She has worked as Research Associate for six month at Department of Biochemistry, Faculty of Medicine, AMU, Aligarh. She also worked as Postdoctoral Fellow of the University Grants Commission, New Delhi since 11 Feburary 2012 to 30 July 2015. She is presently working as Assistant Professor at J.N. Medical College, Aligarh, India. She has attended several national and international conferences and has published 20 papers in international journals.

Abstract

It has been suggested that stress can play a major role in the etiopathogenesis of numerous diseases including diabetes mellitus. Superoxide and nitric oxide are two such stressors produced during inflammation in high amount. They can combine to produce peroxynitrite anion (ONOO-) which is potent oxidizing and nitrating agent. Thus, its interaction with biomolecules can cause oxidation as well as nitration. In the present study, albumin was modified by peroxynitrite and structural changes have been studied by UV, fluorescence, CD and Congo red binding. Analysis of modified-albumin showed increased level of carbonyl, nitrotyrosine and dityrosine. Thiol content was significantly reduced in modified-HSA. Reduction of plasma antioxidant power has been reported in diabetes mellitus and under such conditions peroxynitrite may modify albumin. This may modify the antigenic properties of albumin. Subsequent processing of modified-albumin by immune cells may generate autoantibodies. Thus, peroxynitrite-modified-HSA was used as antigen for detecting autoantibodies in diabetes mellitus sera by ELISA. Peroxynitrite-modified-HSA was bound by the diabetic autoantibodies. The study demonstrates that peroxynitration can generate immunologically active epitope on HSA.