Zeeshan Ansar Ahmed
The Aga Khan University Hospital, Pakistan
Dr. Zeeshan Ansar is currently an Assistant Professor, Molecular Pathology in the Department of Pathology and Laboratory Medicine, Aga Khan University Hospital. He did his MBBS from Dow Medical College in 1999. He has completed his residency in Hematology from the National Institute of Blood Disease and Bone Marrow Transplantation in 2011 and obtained his FCPS in Hematology in 2013. After that he joined Molecular Pathology; Aga Khan University in 2013 and was awarded fellowship in 2014. He then joined the Department as an Assistant Professor in 2015. He has served in the Middle East working as clinical practitioner in Semah Medical Centre, KSA. He has also served senior lecturer in Dow Institute of Heamtology, Dow University of Health Sciences,Karachi.
BCR/ABL fusion gene usually occurs as a result of Philadelphia (Ph)translocation between chromosomes 9 and 22 in Chronic Myeloid leukemia (CML) aswell as in Acute Lymphoblastic Leukemia (ALL). This rearrangement results in the formation a chimeric BCR/ABL fusion gene on the derivative chromosome 22.Fluorescence in-situ hybridization (FISH) analysis using dual color BCR/ABL translocation probes allows the visualization of BCR/ABL rearrangements in both interphase and metaphase cell, and the presence of the BCR/ABL fusion gene on chromosomes 22 has been reported in substantial subset of these patients. The pattern of rearrangement may be classical, variable or mixed. Only classical pattern has been reported to havegood prognosis with lesser disease progression and good response to tyrosine kinase inhibitors.
Methods: The incidence of both classical and variable BCR/ABL gene rearrangement was determined in all the patients suspected of CML and ALL using dual fusion fluorescence