Synchronous fluorescence spectroscopy (SFS) has been found to have several advantages [17], such as simple spectra, high selectivity, low interference, etc. It has attracted the attention of many researchers and developed rapidly since it was firstly proposed by LIoyd. The combination of SFS and derivative synchronous fluorometry (DSF) is more advantageous than differentiation of the conventional direct spectrofluorometry in terms of sensitivity, because the amplitude of the derivative signal is inversely proportional to the bandwidth of the original spectrum .
The normal synchronous fluorescence spectra of ATM and its acidic degradation products were greatly overlapped. This observation led us to utilize first derivative synchronous fluorescence spectroscopy (FDSFS) to separate the drug and its acidic degradation without any interference. The peaks intensities of ATM and the acid degradates were measured at 276 and 265 nm, respectively.
The content of ten capsules of Strattera® was emptied and mixed well. An amount of the powder equivalent to 100 mg of ATM hydrochloride was weighed and transferred into a 100 ml volumetric flask, about 80 ml of water was added and the flask was sonicated for 15 min, mixed well and filtered, then completed to the volume to get 1000 μg/ml stock solution. Then as mentioned in details under each method appropriate working solutions of ATM were prepared and analyzed as described under Construction of Calibration Curve.
Last date updated on April, 2024
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